Recombinant CD3 antibody

Catalog No. ABIN7795298

This Mouse Monoclonal antibody specifically detects CD3 in Func, ELISA, Neut and IGA. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD3 antibody (ABIN7795298)

Target: CD3

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Functional Studies (Func), ELISA, Neutralization (Neut), Immunogenicity Assay (IGA)

Grade: In vivo Grade

Clone: SP34-2

Product Details anti-CD3 Antibody

Expression System: Mammalian cells

Purpose: In vivo Grade Recombinant Anti-human CD3 Monoclonal Antibody (Clone: SP34-2), Mouse IgG1 Kappa

Characteristics: The antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, the antibody, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the antibody, T cells disappear from the circulation within minutes to hours. During treatment with the antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the antibody in vivo. The antibody is ineffective clinically if the CD3 target molecule is not modulated. The antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway